This week we bring you something a little different!
Four eighth grade students from The Nueva School in Hillsborough, California are releasing “They Grow”, a scienceified version of the popular Drake song “Headlines” (warning: Headlines contains explicit language!). In the video the students rap about photosynthesis, starting with the basics and moving on to the intricate processes of the light reaction and the Calvin cycle.
With help from their science teacher, Tom McFadden, these four students wrote and performed their lyrics, then planned, shot and edited their music video. Tom’s “Science Rap Academy” class meets twice a week for an hour each Tuesday and Thursday. The students have been working on this project since January and are very excited to finally release it to the world. They hope that their video will help students better understand the complex process known as photosynthesis.
Watch the video:
More from the students:
“I think that this song and video that we have created will help provide students learning about photosynthesis with a fun, engaging and relatable way to learn how plants grow,” – Alex, coproducer and rapper.
“This song brings energy into the classroom while effectively communicating the perplexing process of photosynthesis. I think that this song will be engaging and entertaining, and was a blast to film,” – Stanley, coproducer and rapper.
“Our number one goal with this song was to make learning about science, specifically photosynthesis, fun to learn. I think that we were able to achieve that by scienceifing a popular song that many kids know, so they can really connect to it. We also made the video and song easy to follow and understand so people of all ages can learn from it,” – Jason, coproducer and filmmaker.
“This entire process was very fun, writing and singing our lyrics and filming the video. I’m really thrilled to share this final product that we have been working hard on to the world and I hope that people enjoy it,” – Quincy, coproducer and rapper.
The Chelsea Flower Show is a world-renowned garden show held every year in May by the UK’s Royal Horticultural Society. There are exhibits of garden design, educational outreach, and exciting new varieties of ornamental plants, fruits and vegetables, with prestigious awards given for each category.
The Chelsea Flower Show, one of the biggest and best known horticultural shows in the world, took place on 24-28th May 2016. Some 150,000 visitors made their way to the Royal Hospital Chelsea, London, UK, to be wowed by innovative garden designs and especially by gorgeous flowers. Among other things, show-goers had the chance to learn the winner of the Royal Horticultural Society’s Plant of the Year award. This annual prize goes to the “most inspiring new plant” on display at the show – a high honor indeed given the number and range of varieties introduced each year.
The relentless pursuit of showy flowers for garden display extends back significantly further than the 104 years of the Chelsea show. One need only recall the infamous Dutch tulip craze of the 17th century to be reminded that fascination with floral novelties has a long and storied history.
Over the centuries, entrepreneurial cultivators have endeavored to create unique plant varieties, either by bringing together the genetic material from established lines through hybridization or through the discovery of new genetic variation such as a chance mutation in a field. Today, flower breeding is pursued with a far better understanding of plant biology than ever before, in some cases with the aid of technologies such as tissue culture and genetic transformation. Yet the goal remains the same: the creation of tantalizing tulips, ravishing roses, show-stopping snapdragons and myriad other plants that will ideally prove irresistible to gardeners and turn a handsome profit.
Chelsea Flower Show 2016. Andy Rain/EPA
The quest to produce profitable new varieties – and to do so as fast as possible – at times led to breeders to embrace methods that today seem strange. There is no better illustration of this than the mid-century output of one of America’s largest flower-and-vegetable-seed companies, W Atlee Burpee & Co.
Gardening with X-rays
In 1941, Burpee Seed introduced a pair of calendula flowers called the “X-Ray Twins”. The company president, David Burpee, claimed that these had their origins in a batch of seeds exposed to X-rays in 1933 and that the radiation had generated mutant types, from which the “X-Ray Twins” were eventually developed.
At the time, Burpee was not alone in exploring whether X-rays might facilitate flower breeding. Geneticists had only recently come to agree that radiation could lead to genetic mutation: the possibilities for creating variation “on demand” now seemed boundless. Some breeders even hoped that X-ray technologies would help them press beyond existing biological limits.
The Czech-born horticulturist Frank Reinelt thought that subjecting bulbs to radiation might help him produce an elusive red delphinium. Unfortunately, the experiment did not produce the hoped-for hue. Greater success was achieved by two engineers at the General Electric Research Laboratory, who produced – and patented – a new variety of lily as a result of their experiments in X-ray breeding.
Though Reinelt’s and other breeders’ tangles with X-ray technology resulted in woefully few marketable plant varieties, David Burpee remained keen on testing new techniques as they appeared on the horizon. He was especially excited about methods that, like X-ray irradiation, promised to generate manifold genetic mutations. He thought these would transform plant breeding by making new inheritable traits – the essential foundation of a novel flower variety – available on demand. He estimated that “in his father’s time” a breeder chanced on a mutation “once in every 900,000 plants”. He and his breeders, by comparison, equipped with X-rays, UV-radiation, chemicals, and other mutation-inducing methods, could “turn them out once in every 900 plants. Or oftener”.
Scientific sales pitches
A 1973 Burpee cover. Burpee, CC BY-NC-ND
Burpee’s numbers were hot air, but in a few cases plant varieties produced through such methods did prove hot sellers. In the late 1930s Burpee breeders began experimentation with a plant alkaloid called colchicine, a compound that sometimes has the effect of doubling the number of chromosomes in a plant’s cells. They exploited the technique to create new varieties of popular garden flowers such as marigold, phlox, zinnia, and snapdragons.
All were advertised as larger and hardier as a result of their chromosome reconfiguration – and celebrated by the company as the products of “chemically accelerated evolution”. The technique proved particularly successful with snapdragons, giving rise to a line of “Tetra Snaps” that were by the mid-1950s the best-selling varieties of that flower in the United States.
Burpee’s fascination with (in his words) “shocking mother nature” to create novel flowers for American gardeners eventually led him to explore still more potent techniques for generating inheritable variation. He even had some of the company’s flower beds seeded with radioactive phosphorus in the 1950s. These efforts do not appear to have led to any new varieties – Burpee Seed never hawked an “atomic-bred” flower – but the firm’s experimentation with radiation did result in a new Burpee product. Beginning in 1962, they offered for sale packages of “atomic-treated” marigold seeds, from which home growers might expect to grow a rare white marigold among other oddities.
Burpee was, above all, a consummate showman and a master salesman. His enthusiasm for the use of X-rays, chemicals, and radioisotopes in flower breeding emerged as much from his knowledge that these methods could be effectively incorporated into sales pitches as from his interest in more efficient and effective breeding. Many of his mid-century consumers wanted to see the latest science and technology at work in their gardens, whether in the form of plant hormones, chemical treatments, or varieties produced through startling new techniques.
Times have changed, 60-odd years later. Chemicals and radiation are as more often cast as threatening than benign, and it is likely that many of today’s visitors to the Chelsea Flower Show hold a different view about the kinds of breeding methods they’d like to see employed on their garden flowers. But as the continued popularity of the show attests, their celebration of flower innovations and the human ingenuity behind these continues, unabated.
On May 18th, botany geeks around the world shared their love of plants in this year’s Fascination of Plants Day! Here’s our round-up of some of the best #fopd tweets!
First things first, test your skills with this challenging plant science quiz:
Farmed fish are often fed with fishmeal, produced from the dried tissues of caught marine fish. In 2012, a total of 16.3 million metric tons of fish were caught to produce fishmeal and fish oil, 73% of which was used in aquaculture. This practice is unsustainable, and as the global human population is expected to rise to 9 over billion by 2050, capture fisheries will not be able to satisfy the demand for fish protein.
Barramundi fish
In recent decades there has been extensive research into ingredients to replace fishmeal, but this has focused mainly on sources of plant carbohydrate and protein such as maize and soy, which also serve as human foods. While these crops are now used in some commercial aquaculture feeds, they are not suitable for many species and have had less than optimal results. In addition, many countries do not grow these mainstream crops and are left in the undesirable position of having to import fishmeal alternatives, which can be cost prohibitive, and increase carbon emissions.
An alternative to fishmeal
Insect based fish feed
The Crops for the Future (CFF) team in Malaysia is working with the University of Nottingham, UK, to investigate insect-based aquaculture feed as a replacement to fishmeal use in fisheries. Both organizations recognize that current rates of wild fish depletion are unsustainable and will not meet future demand for fishmeal under a ‘business as usual’ scenario. With support from the Newton-Ungku Omar Fund Institutional Linkages Programme, they have shown that the quality of insect larvae as an aquafeed ingredient is affected by the substrate on which the insects feed.
The CFF ‘FishPLUS’ program has revealed that black soldier fly (BSF; Hermetia illucens) larvae fed with underutilized crops can be used to produce insectmeal and replace up to 50% of fishmeal in formulated aquaculture. These crops are not used for human food and can be grown on marginal land close to areas of aquaculture production in tropical climates, increasing the sustainability of the process.
Producing insectmeal with underutilized crops
Ground Sesbania is used to feed the black soldier fly larvae
Over a year, the researchers worked with a private sector supplier to develop laboratory-scale BSF breeding pods in which different substrate combinations of underutilized crops could be trialed. BSF feeding trials were conducted using five separate or combined underutilized crops as substrate, i.e. Sesbania (Sesbania sp.); 90% Sesbania with 10% Moringa (Moringaoleifera); Bambara groundnut (Vigna subterranea) leaf; Bambara groundnut flour; and Moringa leaf.
The best results were obtained by feeding the larvae on Sesbiana, a nitrogen-fixing legume that grows well in marginal tropical landscapes and is not a human food crop. Overall, nutrient analyses indicated that the amino acid profile for insectmeal is encouraging and closely resembles fishmeal.
Successful feeding trials
Black soldier fly larvae
Fish feeding trials using the BSF insectmeal were undertaken in Malaysia at the CFF Field Research Centre. The trial fish, barramundi, accepted a formulated feed with up to 50% replacement of fishmeal with Sesbania-fed BSF insectmeal. The feed conversion ratio, mortality rate and biomass growth rate were all comparable to control trials with commercial fishmeal aquaculture feed. Back in the UK, complementary antinutritional studies at the University of Nottingham contributed essential information to guide the development of an optimal aquaculture feed formulation in the future.
Waste not, want not
Amaranth growing with either commercial fertilizer (right) or FishPLUS substrate compost (left)
This project also embraces the use of undigested material from the insect feeding as compost for crops like okra and amaranth. For example, 10kg of Sesbania leaves produces 1kg of BSF pre-pupae and 9kg of undigested waste material. When used as a soil conditioner in our agronomy trial, this waste material improve the crop growth at a comparable level to commercial fertilizer. This could be used by terrestrial crop farmers to reduce their fertilizer bill.
The findings of this project are of importance to world food security. As leaders in this field of research, the UK and Malaysian partners are well placed to leverage these preliminary results and explore scalability and options for commercialization of benefit to both economies.
CFF is the world’s first and only organization dedicated to research on underutilized crops. Professor M.S. Swaminathan, World Food Prize Laureate and Father of the Asian Green Revolution, described CFF as `the need of the hour.’
You can see more about the FishPLUS project from Crops for the Future in the video below:
This article was written by FishPLUS Team, for Crops for the Future.
Considering its weedy nature, Arabidopsis thaliana is a fussy little plant. This can be a pain – even tiny environmental fluctuations can have significant impacts on the physiology and development that many of us are investigating.
As silly as it sounds, my labmates and I have spent many months debating the best compost media to use when growing Arabidopsis for research. It began when our trusted compost supplier changed the formula of its peat-based compost, which stressed our plants and turned them a lovely shade of purple! The conversation has continued to develop as we learn about the different media used in other laboratories.
A new paper from Drake et al. at my university (University of Bristol, UK) has added a new depth to the debate, so I thought I’d bring it all to your attention and perhaps receive some other suggestions to consider!
Peat-based vs non-peat compost
Arabidopsis growth on peat-based and peat-free growth media. Drake et al., 2016.
The experiment, led by Dr Antony Dodd, was designed to test whether peat-based composts could be replaced by alternatives in Arabidopsis research, in an attempt to reduce plant science’s use of unsustainable peat extraction. The researchers grew two ecotypes of Arabidopsis (Col-0 and Ler) on both autoclaved and non-autoclaved composts, including peat-based compost and some formed of coir, composted bark, wood-fiber, and a domestic compost.
In terms of reducing peat use, Arabidopsis unfortunately grew best on the peat-based growing media, although some vegetative traits were comparable in some peat-free composts.
Autoclaving compost
This study caught my eye for another reason, however. We always sterilize our compost before growing Arabidopsis to reduce its contamination by fungi and insect pests; however, after learning that manganese toxicity can become a problem, we no longer autoclave it. As you can see in Boyd’s 1971 paper, manganese is converted to a more bioavailable form during the autoclave process, which can be toxic to plants.
Interestingly, Drake et al.’s research revealed no differences in Arabidopsis growth on autoclaved vs. non-autoclaved media, but I expect that in other environmental conditions the elevated manganese availability could become a problem. They did find that the autoclaved soil actually had more issues with mildew and algae, possibly because the natural microbiota had been killed and the compost was therefore easier to colonize.
Insecticide treatment
One of the biggest issues our lab has with non-autoclaved soil is the presence of small insects, which can predate our precious plants. A potential alternative to autoclaving is to treat the media with insecticide, such as imidacloprid, a neonicotinoid. However, many labs have stopped using these pesticides; in 2010, Ford et al. showed that several neonicotinoids, including imidacloprid, induce salicylate-associated plant defense responses associated with enhanced stress tolerance, while in 2012, Cheng et al. found 225 genes were differentially expressed in rice plants treated with imidacloprid. In experiments designed to measure precise physiological responses, I’m not convinced that it’s a good idea to use these pesticides!
Potential alternatives
To avoid using autoclaves and insecticides, you could consider baking compost overnight at 60°C (140°F) to try and kill fungal spores and insects, freezing the media, and/or using biocontrols to tackle insect pests, such as nematodes or mites.
In the peat vs. non-peat debate, it looks as though peat-based media are still the frontrunners in terms of compost, but hydroponic systems are becoming more popular as a way of tightly controlling nutrient regimes and manipulating whole plants more easily. Check out this video from Associate Professor Matthew Gilliham (University of Adelaide, Australia) to learn more about the technique:
If you have any other suggestions, please leave a comment and share your methods and ideas!
In June 2016, the UK Government will hold a public referendum for the people to decide whether or not Britain should exit the European Union. This contentious issue, popularly known as “Brexit”, has even divided the governing political party, with key parliamentary figures standing on either side of the debate.
There are many complex political issues for the UK to consider ahead of this referendum. One of these issues is: “what would be the consequences for UK agriculture if Britain were to leave the EU?” Professor Wyn Grant, a member of the Farmer–Scientist Network in the UK, tells us about a new report asking this very question.
Brexit and agriculture
by Wyn Grant
The Farmer–Scientist Network was set up by the Yorkshire Agricultural Society (UK) to facilitate practical cooperation between farmers and academics on the challenges facing agriculture. The Network felt there was a need to produce an assessment of the possible consequences of Brexit for agriculture. A working party was established, made up of leading experts on the EU’s Common Agricultural Policy and farmer members. I chaired this working party, and we produced what we hope is a comprehensive report, available here: http://yas.co.uk/charitable-activities/farmer-scientist-network/brexit.
In producing the Brexit report, one of our objectives was to provide information that farmers and others concerned with agriculture could use to question politicians during the referendum campaign. We also felt that agriculture and food had not been given sufficient attention during the negotiations and subsequent discussions. Should Brexit occur, our report draws attention to the issues that would have to be considered in exit negotiations.
The ins and outs of leaving
When evaluating the implications of Brexit for agriculture, we expected there would be complexities and uncertainties, but these were, in fact, greater than we anticipated. One reason for this is that, although the Lisbon Treaty on which the EU is founded makes provision for Member States to leave the EU under ‘Article 50’, none have ever done so before. It is difficult to know in advance how Britain’s exit would proceed, but it would almost certainly be necessary to use the entire two-year negotiating window provided for in the Treaty. Another complication is that the UK Government has not undertaken any formal contingency planning for exit, so it is difficult to know what a future domestic agricultural policy would look like.
In the event of Brexit taking place, the Farmer–Scientist Network feels that an optimal arrangement for the UK would be to establish a free trade area with the rest of the EU, with tariff-free access for UK farm products to the internal market. However, we don’t think the EU would want to give too generous a deal for fear of encouraging other member states to think about the benefits of exit.
Subsidies
Currently, two ‘pillars’ of financial subsidy are awarded to stakeholders in EU agriculture. We believe that the existing ‘Pillar 1’ subsidies that are given to EU farmers would be vulnerable after Brexit. This is an important issue, as for many farmers these subsidies make the difference between making a profit and running at a loss. Supporters of Brexit argue that the savings made from contributions to the EU budget would more than allow for subsidies to continue to be paid at the existing level. However, this overlooks the fact that the UK Treasury has for a long time targeted these subsidies as “market distorting”, and in the current climate of austerity in the UK, they could be at risk of being phased out as a means to reduce public expenditure.
We did, however, think that the ‘Pillar 2’ subsidies directed at agri-environmental and rural development objectives would be continued in some form. This is in part because they are embedded in contracts that continue beyond 2020, and because they have a coalition of domestic support from outside the industry from environmental and conservation lobbies.
Regulation
Some farmers resent what they see as excessive regulation emanating from Brussels. However, we think it is unlikely that many of these controls would be dropped or relaxed following Brexit. There are good reasons for regulations covering such areas as water pollution, pesticide use and animal welfare that have nothing to do with membership of the EU. Domestic support for such regulations would continue from environmental, conservation, public health, animal welfare and consumer organisations.
Some farmers hope that plant protection products that have been banned under EU regulations could be used after Brexit. However, there would still be domestic pressure to regulate these products and manufacturers might be unwilling to produce them just for the UK market.
Negotiation and trade
The UK at present negotiates in the World Trade Organisation (WTO) as a part of an EU bloc which provides additional leverage against powerful countries such as the United States. The agreements that the EU has with ‘third’ countries (those outside of Europe) would have to be renegotiated on a single country basis. Supporters of Brexit are confident that this task could be completed within two years. However, given that the UK has relied on the negotiating resources of the European Commission, it does not have many international trade diplomats and the process could take considerably longer.
Migrant labour
The horticulture industry in the UK is substantially dependent on migrant labour from elsewhere in the EU. This could not easily be replaced with domestic labour. It would be necessary to try and negotiate a new version of the Seasonal Agricultural Workers Scheme (SAWS) – a scheme (redundant since 2013) that was established to allow migrant workers from certain countries outside of the EU to work in UK agriculture – to ensure that the sector would have the labour it needs to function.
Conclusions
Being part of a larger political community gives British farmers some political cover from countries where farming makes up a large share of GDP or has strong cultural roots. The Farmer–Scientist Network concluded that it was difficult to see Brexit as beneficial to UK agriculture. However, we also emphasised that there are broader considerations about UK membership that needed to be weighed in any voting decision.
It is a well known fact that biologists are a clever bunch. Most of the time they’re out applying their intellect and tackling the world’s problems, but occasionally (probably at happy hour on a Friday evening) they sit around coming up with witty names for genes.
Don’t worry though – plant scientists have come up with some clever gene names of their own! I asked the #plantsci community on Twitter for their favorites:
We all know that #plantsci-entists make the best comedians. Anyone got a favourite funny gene name? I like SUPERMAN and KRYPTONITE
Thesupermanmutant in Arabidopsis lacks the female parts of the flower, replacing it with more stamens. Fairly funny on its own, but naming its suppressor KRYPTONITEwas even better!
@JoseSci KOJAK and WEREWOLF are two of my favourites — Jenny Mortimer (@Jenny_Mortimer1) March 31, 2016
Like the 1970s TV cop Kojak, the kojakmutant is completely (root) hairless! In contrast, the werewolf mutant produces LOTS of root hairs.
Ah yes, we can partially blame GPC’s Ruth Bastow for this one as she was co-first author on the discovery paper! TIMING OF CAB EXPRESSION1 (TOC1) had been shown to be involved in the circadian clock, and when Ruth and her colleagues discovered a gene that appeared to regulate TOC1, they named it TIC for the clever TIC-TOC of the circadian clock, then fit the full name (TIME FOR COFFEE) around it! The official reason was, “We located TIC function to the mid to late subjective night, a phase at which any human activity often requires coffee”. Hmm!
My thesis is on stomatal development, so these are close to my heart! The word ‘stoma’ is ancient Greek for ‘mouth’, so lots of stomata genes are mouth-based puns!
Where does YODAfit into this, you ask? This gene is the (Jedi) master regulator of stomatal development, of course!
— Christopher Grefen (@PlantesTrouveur) April 1, 2016
In the run-up to the Brexit referendum on the United Kingdom leaving the European Union, SCHENGEN is a topical choice! This gene is involved in establishing the Casparian Strip, a lignified type of cell wall located in the endodermis. The schengen mutants don’t form this barrier, so were named after the Schengen Agreement that ‘established a borderless area between European member states’.
Lisa’s spot on with these. The pennywise mutation was discovered first, named after a band, then when a paralogous gene was identified by the same authors, they continued the finance theme with POUND-FOOLISH.
@IHStreet@JoseSci well we are very fond of the ‘dillos (ARABIDILLO, PHYSCODILLO, etc) in my lab but we are biased…. — Juliet Coates (@JulietCCoates) April 6, 2016
The armadillo mutant in Drosophila has abnormal segment development, which looks a little like the armor plating of an armadillo. This protein contains ‘Armadillo repeats’, which is actually found in a huge variety of species including plants. The ARABIDILLO genes in Arabidopsis promote lateral root development, while PHYSCODILLO genes affect early development in the moss Physcomitrella patens.
.@JoseSci Not meant to be funny, but ARFs, because of their onomatopoetic nature are funny. — Ian Street (@IHStreet) April 5, 2016
.@JoseSci And for similar reasons: ARRs! Pirate sounds are always funny. — Ian Street (@IHStreet) April 5, 2016
Thanks, Ian!
Thanks to everyone who participated in this list. If you have a favorite whimsical gene name that hasn’t been mentioned, let us know in the comment section!
The Atacama Desert is a strip of land near 1000 km in length located in northern Chile. With an average yearly rainfall of just 15 mm (close to 0 in some locations) and high radiation levels, it is the driest desert in the world. Geological estimates suggest that the Atacama has remained hyperarid for at least eight million years. Standing in its midst, one may easily feel as though visiting a valley on Mars.
Despite these harsh environmental conditions, it is possible to find life in the Atacama. At the increased altitudes along the western slopes of the Andes precipitation is slightly increased, allowing plant life.
Convergent evolution
The driest and oldest desert in the world acts as a natural laboratory where for 150 million years plants adapted to and colonized this environment. These adaptations are likely present in multiple desert plant lineages, thus providing an evolutionary framework where these traits can be associated with a signature of convergent evolution.
Surviving a nitrogen-limited landscape
Image credit: Center for Genome Regulation
The interplay of environmental conditions in the transect of the Atacama, ranging from 2500 to 4500 meters above sea level, results in three broad microclimates; Pre Puna, Puna, and High Steppe. These microclimates have different humidities, temperatures, levels of organic matter and even different pH levels, but share one common feature: low nitrogen levels.
To engineer crops with higher nitrogen use efficiency, it is very useful to first learn how plants adapt to growth in low nitrogen environments. Here the Atacama Desert enters into the game. Plants growing in the desert can survive 100-fold less nitrogen below optimum concentrations. Using phylogenetics it is possible to uncover novel genes and mechanisms related to adaptation to these extreme conditions, which have not been discovered through traditional genetic approaches.
Currently, nitrogen fertilizers are widely employed to increase crop yield. In 2008 100 million tons of this fertilizer were used and it is projected that for 2018 the demand for nitrogen will rise to 119 million tons. Regretfully, the production and over-usage of this type of fertilizer has an enormous impact in the environment and human health. Around 60% of the nitrogen introduced to the soil for agricultural purposes is leached and lost. Moreover, nitrogen runoffs to the water cause eutrophication in both freshwater and marine ecosystems, leading to algae and phytoplankton blooms, low levels of dissolved oxygen, and finally the migration or death of the present fauna, forming dead zones such as the one in the Gulf of Mexico.
Image credit: Center for Genome Regulation
Nitrogen fertilizers are not the only major concern in modern agricultural procedures. The co-localization of drought and low nitrogen levels is especially detrimental for plant growth and development. We need to support not only the nutritional requirement of an expanding global population but also new energetic strategies based on production of biomass for biofuels on marginal nutrient poor soils. In order to increase crop yields while reducing the environmental impact of nitrogen fertilizers, it is necessary to develop new agricultural strategies and cutting edge technologies.
Learning from the desert
What if we could profit from the extraordinary plants that have had thousands of years to learn how to cope with nitrogen scarcity, drought and extreme radiation? Specifically, can we unravel the genes and mechanisms that allow them to survive in such a barren place?
Image credit: Center for Genome Regulation
Over the past three years our group has identified 62 different plant species that inhabit the Atacama Desert, and established a correlation between their habitat attributes and biological characteristics. Using tools such as whole transcriptome shotgun sequencing or RNA-Seq complemented with different bioinformatics approaches, we have identified over 896,000 proteins that are expressed in these conditions.
In this way we aim to learn which processes are highly utilized in these “extreme survivors” compared to similar species that are present in the deserts of California, where the climatic conditions are similar but there is no nitrogen scarcity. That is how we expect to find new mechanisms (or, more precisely, very old mechanisms) that enable plants to survive and grow efficiently in extreme environments.
Last April I joined the Global Plant Council as a New Media Fellow along with Sarah Jose from the University of Bristol. The GPC is a small organization with a big remit: to bring together stakeholders in the plant and crop sciences from around the world! As New Media Fellows, Sarah and I have have assisted in raising the online profile of the GPC through various social media platforms. We wrote about our experiences in growing this blog and the GPC Twitter and Facebook accounts in the The Global Plant Council Guide to Social Media, which details our successes and difficulties in creating a more established online presence.
Why do it?
My wheat growing in Norfolk field trials. I have spent every summer for the past 3 years out here analysing photosynthesis and other possible contributors to crop yield
I chose to apply for the fellowship during the third year of my PhD. Around this time I had started to consider that perhaps a job in research wasn’t for me. It was therefore important to gain experience outside of my daily life in the lab and field, explore possible careers outside of academia and of course to add vital lines to my CV. I still loved science, and found my work interesting, so knew I wanted to stay close to the scientific community. Furthermore, I had always enjoyed being active on Twitter, and following scientific blogs, so the GPC fellowship sounded like the perfect opportunity!
The experience
I think I can speak for both Sarah and myself when I say that this fellowship has been one of the best things I’ve done during my PhD. Managing this blog for a year has allowed me to speak to researchers working on diverse aspects of the plant sciences from around the world. My speed and writing efficiency have improved no end, and I can now write a decent 1000 word post in under an hour! I discovered the best places to find freely available photos, and best way to present a WordPress article. Assisting with Twitter gave me an excuse to spend hours reading interesting articles on the web – basically paid procrastination – and I got to use my creativity to come up with new ways of engaging our community.
Filming interviews at the Stress Resilience Forum. Next career move, camera woman?
Of course going to Brazil for the Stress Resilience Symposium, GPC AGM and IPMB was a highlight of my year. I got to present to the international community both about my own PhD research and the work of the GPC, Sarah and I became expert camera women while making the Stress Resilience videos, and I saw the backstage workings of a conference giving out Plantae badges on the ASPB stand at IPMB. It didn’t hurt that I got to see Iguassu Falls, drink more than a few caipirinhas and spend a sneaky week in Rio de Janeiro!
Helping out on the ASPB stand with Sarah
Thank you
Working with the GPC team has been fantastic. I learnt a lot about how scientific societies are run and the work they do by talking to the representatives from member societies at the AGM. The executive board have been highly supportive of our activities throughout. Last but not least, the lovely GPC ladies, Ruth, Lisa and Sarah have been an amazing team to work with – I cannot thank you enough!
I have now handed in my PhD, left the GPC, and moved on to a new career outside of academic research. I’m going into a job focused on public engagement and widening access to higher education, and have no doubt my GPC experiences have helped me get there. My advice if you’re unsure about where you want to end up after your PhD? Say “yes” to all new opportunities as you never know where they will take you.
Thank you the GPC! Hopefully I’ll be back one day!
It felt particularly fitting to travel to a plant science workshop in Japan, home of hanami, the ancient celebration of the beauty of flowers. Researchers from Heidelberg University, Germany; Kyoto University, Japan; and the University of Bristol, UK, met to discuss their cutting edge research in a meeting aptly titled ‘Novel Frontiers in Botany’.
Plant science at all three of these universities is enhanced by their botanic gardens. Heidelberg’s sounded especially impressive: established in 1593, it boasts over 10,000 species! As a former employee of the Royal Botanic Gardens, Kew (UK), which was recently threatened with a £5 million cut in government spending, I started thinking how important botanic gardens such as these are as vital tools for research, conservation, education and recreation.
Research
Kyoto University Botanic Gardens. Image credit: Sarah Jose
Botanic gardens are focused pools of amazing expertise in horticulture, taxonomy and ethnobotany, and this knowledge is constantly growing. Plant collections at Kyoto, Heidelberg and Bristol botanic gardens are continuously being visited by researchers studying almost all aspects of plant science. Some of the rarest species in the world can be cultivated and studied in botanic gardens without damaging wild populations; others are investigated for potential medicinal properties, evolution is studied by building banks of DNA, and we can get a fantastic overview of the diversity of life, from the microscopic structures of pollen to the architecture of trees.
Ex situ conservation
In the same way that zoos protect endangered species, botanic gardens around the world host and preserve at least a third of all known species of flowering plants.
Wollemi pine pollen cone. Image credit: Velella. Used under license: CC BY-SA 3.0.
One of my favorite examples is the wollemi pine (Wollemia nobilis). Plant nerds like me will know that the wollemi pine was only known from 2 million-year-old fossils until field officer David Noble discovered around 100 plants growing at Wollemi National Park, Australia. These living fossils were propagated by botanic gardens in Australia and internationally, and are now a popular tree in cultivation around the world.
Another important facet of ex situ conservation is the creation of seed banks. The Royal Botanic Gardens, Kew, established the Millennium Seed Bank in 1996, which aims to provide an ‘insurance policy’ against extinction by storing the seeds of 25% of all seed-bearing plants. The majority of the stored 75,000 species will be rare or threatened with extinction.
In situ conservation
As well as bolstering plant species on their own sites, many botanic gardens are involved in restoration projects of damaged ecosystems around the world. Botanic Gardens Conservation International, a global network for botanic gardens, describes many such efforts including the restoration of Brazil’s Atlantic Forest by the Rio de Janeiro Botanic Garden, the restoration of local upland forest by Kenya’s Brackenhurst Botanic Garden, and Hawaiian beach and coastal restoration by the US National Tropical Botanic Garden. In each case, rare and endemic species of plant replaced invasive species and increased the biodiversity of the entire ecosystem.
Education and recreation
Botanic gardens often hold events, like the Easter sculpture festival at the University of Bristol Botanic Gardens. Image credit: Sarah Jose
Botanic gardens are a great place for an afternoon stroll with the family, which plays nicely into their ulterior motive: education. Plants are amazing but they are often overlooked. By displaying the most wonderful and fascinating species from around the world, botanic gardens kindle an interest in plants from the general public, as well as potentially teaching children where food comes from, or fostering a love of nature and the environment.
It doesn’t stop there though. As well as restoration projects, which often include local people who learn about the economic importance of preserving their native ecosystems, the gardens can be involved in broader efforts. For example, the National Museums of Kenya are looking to develop a botanic garden. The garden will facilitate the transfer and accumulation of ethnobotanical knowledge surrounding the relationships between the Kenyan people and their local plant species; useful information that will also promote the importance of conserving their local flora.
In conclusion
Botanic gardens are integral to the study and appreciation of a wide range of plant taxa, and their importance should not be underestimated. Too many people view them as outdated and push to cut their funding, but we need to show that dynamic and cutting edge research, as well as public appreciation for plants and the environment, relies on their continuation both now and in the future.
This week we bring you something a little different!
