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A Postcard From… The Australian Society of Plant Scientists

By | Blog, GPC Community

A new feature on the Global Plant Council blog will be ‘A Postcard From…’ In these posts representatives from our member organizations will tell us about their society’s visions, aims and activities.

This pioneering ‘Postcard From’ was sent in by Gonzalo Estavillo and John Evans, both members of    the ASPS.

aspspostcard

The Australian Society of Plant Scientists (ASPS) promotes plant science in Australia, and provides professional contact within our community of teachers and researchers in plant biology. Originally fo­unded in 1958, the ASPS currently has approximately 400 members from Australia and also overseas. It provides a forum for knowledge exchange so that the membership can build on both the depth and breadth of knowledge of plant functions. ASPS offers a unifying representation of plant scientists in Australia, and is linked with the Global Plant Council and many other important international plant science organizations.

One of the main activities of the ASPS is to provide mutual support and collective mentorship to facilitate the dissemination of new research. For example, there has been a long and mutually supportive interaction between ASPS and Functional Plant Biology, which is perhaps the most prestigious journal of the Commonwealth Scientific and Industrial Research Organisation (CSIRO). ASPS is one of the foundation partner societies of ComBio, the annual international biology conference held in Australia. ASPS also sponsors other specialist workshops upon request.

The Society aims to nurture a new generation of plant scientists in many ways. It sponsors student attendance to ComBio through travel awards, and encourages active student participation by awarding poster prizes. Additionally, the RN Robertson Travelling Fellowship is available to students and early career researchers to support their research in another laboratory so as to widen their experience and raise their profile.

ASPS rewards excellence at all levels of scientific career development. Eminent plant scientists are invited to give the JG Wood or RN Robertson lectures at ComBio, in honour of the first two Presidents of the Society. Outstanding young plant scientists are recognized every year by the Peter Goldacre Award and the ASPS–FPB Best Paper Award. The commitment of ASPS to plant science education is reflected by both the ASPS Teaching Award, which recognizes innovative contributions to undergraduate teaching, and the development of online resources for plant biology teaching such as Plants in Action.

The Society’s social media platforms work with members to enhance their ability to do research and to educate others in plant sciences. The ASPS website offers the opportunity to connect with other members, get updates on the latest plant science research around the world, post jobs, student scholarship opportunities and conference announcements, and provides a growing collection of teaching resources for plants sciences. Phytogen is the Society’s newsletter blog to inform our own members and general readers with an interest in developments in Australian plant science, provide a vehicle for communicating new ideas, recent professional experiences, and forthcoming events. Finally, we use our Facebook and Twitter (@asps_ozplants) accounts to interact and engage with both scientific and general audiences. Meet us and view our photos in our ASPS Facebook page!

John Evans

John Evans is the current President of the ASPS and researches the physiology of photosynthesis at the Australian National University

Gonzalo

Gonzalo Estavillo is currently a research scientist at CSIRO and tweets @GMEstavillo

 

 

 

 

 

 

 

 

The Nature of Crop Domestication

By | Blog, Global Change

Why do we eat some plants but not others? What makes a good crop, and how have we transformed these species to suit our own needs?

Around 12,000 years ago, humans began to transition from nomadic hunter-gatherer societies to a more settled agricultural life. We began to selectively breed cereals and other crops to improve desirable traits, such as their yields, taste and seed retention. Today we eat less than 1% of all flowering plant species, relying on a handful of staples for almost all of our calories.

Why do we eat so few plant species?

Professor John Warren, Aberystwyth University

Professor John Warren, Aberystwyth University

We spoke with Professor John Warren at Aberystwyth University in the UK, who delves into the history of crop domestication in his new book, ‘The Nature of Crops: How We Came to Eat the Plants We Do,’ published on 24th April 2015. He blogs about how we came to eat certain plants over at Pick of the Crop, and said that his book developed from there. “The stories of crop domestication are just so interesting, weird, biologically strange, fun – they just demand to be told,” he enthused.

So why do we eat so few of the edible plants in the world? Based on his research into gene flow and plant breeding systems, Professor Warren presents novel theories in his book: “Previously people have argued that it’s because most plant are poisonous, but I don’t think that holds water. We actively seek out toxic plants as crops; plants with large food stores tend to be well defended with toxins. Instead I argue that it’s plant sexual habits that limit crop domestication. Plants with the usual pollination mechanisms don’t make ideal crops as they will fail to set seed when grown on an agricultural scale. Thus we domesticate things that are wind pollinated or pollinated by common generalist insects.”

Science-led crop breeding

Why do we eat poisonous plants?

How did our ancestors come to realise that rhubarb leaves are poisonous but the stems make a tasty crumble? Professor Warren says, “Its discovery was an accident and a fairly recent one – but read the book for the full story.” Image credit: Cory Doctorow used under CC BY-SA 2.0.

Professor Warren works at the Institute of Biological, Environmental and Rural Sciences (IBERS) at Aberystwyth University, which houses much of the research into agriculture and the environment that ties into the theme of his book. “Previously it’s been argued that there haven’t really been any new crops in the last 5,000 years. Here in Aberystwyth, we know that ryegrass, clover, elephant grass and others are still in the process of being domesticated, so you don’t need to be an archaeologist to study the process,” he explained. In addition to breeding new varieties of cereals and forage crops for food and feed, the Public Good Plant Breeding group at IBERS are also in the process of breeding Miscanthus, a fast-growing grass species that could be used for sustainable bioenergy in the future.

Resources like the Diversity Seek (DivSeek) initiative, established by the Global Plant Council in association with the Global Crop Diversity Trust, the CGIAR Consortium and the Secretariat of the International Treaty on Plant Genetic Resources for Food and Agriculture, could be used to enable science-driven crop breeding and domestication. DivSeek aims to unlock the genetic diversity that is currently stored in genebanks around the world by using cutting edge sequencing, phenotyping and ‘big data’ technologies. The genetic variation that is identified can then be used as the basis for breeding programs and could assist in the domestication of novel crops.

The future of agriculture

Drought damage

Drought damage in California, 2014. Image credit: US Department of Agriculture used under CC BY 2.0.

The crops we eat today were domesticated in highly fertile conditions; this means they are nutritious but tend to demand a high input of fertilizers and water. Professor Warren argues that we can use modern science to develop more sustainable ways to feed the global population: “It’s important that we start to think outside the box and try and domesticate a whole range of new crops that are more sustainable and less demanding of agricultural inputs.” An important source of future crop species could be stress-tolerant plants living in difficult environments: “I think the crops of the future could still be waiting to be domesticated from plants growing in harsh conditions,” explained Professor Warren.

Professor Warren also discussed how we could use underutilized crops in new ways to make agriculture more sustainable in the future: “I think and hope that we will eat more species, and that we will grow many more of these as perennials in poly-culture systems. That makes ecological sense in terms of niche exploitation and yield sustainability. It also makes more genetic sense in terms of resistance to pests and diseases.” The only downside, he said, is that these systems are so different to what we have now that we will need innovative research to develop them.


About Professor John Warren

Akee fruit

The akee is the national fruit of Jamaica. Image credit: Loren Sztajler, used under CC BY-ND 2.0.

John is a plant ecologist at Aberystwyth University, UK, with research interests in the origin and maintenance of diversity and enhancement of conservation value, particularly within agricultural ecosystems. He is the Director of Teaching and Learning and a Professor of Botany in the Institute of Biological, Environmental and Rural Sciences. John says the strangest plant he’s ever eaten is the akee, a plant beloved of Jamaicans that looks and tastes a bit like scrambled eggs but which is delicious with saltfish.


Over to you

What do you think will be the most important crops of tomorrow, and which underutilized plants will become dietary staples in an effort to feed the world more sustainably?

The Next Generation

By | ASPB, Blog, Future Directions, GPC Community, SEB

Meet Amelia and Sarah, the two newest additions to the Global Plant Council team.

As a coalition of plant and crop societies from the around the globe, the Global Plant Council (GPC) aims to bring together scientists, policy makers and other stakeholders to engage in coordinated strategies to find solutions to global problems.

The GPC currently has 29 member organizations representing thousands of scientists in diverse disciplines around the world. Online media such as this blog and the @GlobalPlantGPC Twitter account provide a fantastic resource for our member organizations to stay in touch, share ideas and develop interdisciplinary collaborations.

For Spanish speakers, we’ve also recently launched a Spanish version of our Twitter feed at @GPC_EnEspanol, kindly translated for us by Juan-Diego Santillana-Ortiz, an Ecuadorian currently studying at Heinrich-Heine University in Düsseldorf, Germany.

Amelia is in the third year of her PhD at the John Innes Centre, Norwich UK. She is researching how altering the biochemistry of epicuticular waxes affects the physiology and ultimately yield of UK wheat. She tweets @AmeliaFrizell (https://twitter.com/AmeliaFrizell)

Amelia Frizell-Armitage is in the third year of her PhD at the John Innes Centre, Norwich UK. She is researching how altering the biochemistry of epicuticular waxes affects the physiology and ultimately yield of UK wheat. She tweets @AmeliaFrizell.

To further enhance this network, the GPC has awarded two New Media Fellowships to early career plant scientists Amelia Frizell-Armitage and Sarah Jose. The role of the Fellows will be to increase visibility of the GPC through managing this blog, devising new strategies to promote GPC activities and to increase traffic flow and engagement on Twitter.

A key priority will be to increase members’ contributions to this blog to promote their organizations and associated activities. Contributing to the blog is a fantastic way to interact with other GPC members, and we are always open to suggestions for guest posts. Perhaps you want to talk about a recent meeting or activity, discuss a particularly exciting piece of emerging research, promote a newly published book, or even just give some insight into your everyday life?

Sarah Jose is a third year PhD student at the University of Bristol, UK. She is investigating the link between wax biosynthesis and stomatal development in barley and Arabidopsis, and its potential impact on the water use efficiency of plants. Find her on Twitter @JoseSci.

Sarah Jose is a third year PhD student at the University of Bristol, UK. She is investigating the link between wax biosynthesis and stomatal development in barley and Arabidopsis, and its potential impact on the water use efficiency of plants. Find her on Twitter @JoseSci.

Whatever it is, we want to hear from you! Please get in touch on Twitter, via the comments section on the blog, or by emailing our Outreach & Communications Manager Lisa Martin.

It is an exciting year ahead for the GPC with the launch of a new online platform for the plant community that is being built in partnership with the ASPB and with support from SEB. There are also various fundraising initiatives in the works, and a Stress Resilience Forum coming up in October, which is being organized in collaboration with SEB.

Stay tuned to this blog to keep up to date with all our activities. The events calendar for member organizations is also looking busy and vibrant, and can be found here.

 

 

 

 

 

 

 

 

 

 

Can you crowdfund the sequencing of a plant genome?

By | Blog, Future Directions, Global Change
Dr Peng Jiang, University of Georgia, USA

Dr Peng Jiang, University of Georgia, USA

Peng Jiang and Hui Guo at the University of Georgia think you can! They are currently raising money via a crowdfunding approach to sequence the first cactus genome – but the question is: why would they want to? Peng explains all in this guest blog post.

A Prickly Proposal: Why Sequence the Cactus?
In these times of growing food insecurity due to climate change and population pressures, the prickly pear cactus (Opuntia ficus) has growing commercial and agricultural importance across much of the world – you will find it growing in Mexico and Brazil, Chile, large parts of India and South Africa, and in Spain and Morocco.

The goal of our proposal is to sequence the genome and transcriptome of the prickly pear cactus, a recognized food and forage crop in these challenging semiarid regions of the world.

With more than 130 genera and 1,500 species of Cactaceae, we will create a draft genomic and transcriptome database that would aid the understanding of this understudied plant family, and provide the research community with valuable resources for molecular breeding and genetic manipulation purposes. Here are some of the reasons why we think a first cactus genome would be so important:

The Prickly Pear Cactus

The Prickly Pear Cactus

1. Ecological Improvement
The beauty of the drought-tolerance cactus is that it can grow on desert-like wastelands. Nowadays, more than 35% of the earth’s surface is arid or semiarid, making it inadequate for most agricultural uses. Without efforts to curb global warming, “Thermageddon” may hit in 30–40 years time, causing desertification of the US, such that it may become like the Sahara. Opuntia helps create a vegetative cover, which improves soil regeneration and rainfall infiltration into the soil. This cactus genome research may help us to adapt our food crops to a much hotter, drier climate.

2. Food Crops, Feed and Medicine
The fruits of prickly pear cactus are edible and sold in stores under the name “tuna”. Prickly pear nectar is made with the juice and pulp of the fruits. The pads of prickly pears (“Nopalito”) are also eaten as a vegetable. Both the fruits and pads of prickly pears can help keep blood sugar levels stable because they contain rich, soluble fibers. The fruit contains vitamin C and was used as an early cure for scurvy.

Furthermore, there has been much medical interest in the prickly pear plant. Studies [1, 2, 3] have shown that the pectin contained in prickly pear pulp lowers cholesterol levels. Another study [4] found that the fibrous pectin in the fruit may lower a diabetic’s need for insulin. The plant also contains the antioxidant flavonoids quercetin, (+)-dihydroquercetin (taxifolin), quercetin 3-methyl ether (isorhamnetin) and kaempferol, which have a protective function against the DNA damage that leads to cancer.

3. Biofuels in Semiarid Regions
Planting low water use, Crassulacean acid metabolism (CAM; a water saving mode of photosynthesis) biofuel feedstocks on arid and semiarid lands could offer immediate and sustained biogas advantages. Opuntiapads have 8–12% dry matter, which is ideal for anaerobic digestion. With an arid climate, this prevents the need for extra irrigation or water to facilitate the anaerobic digestion process. Requiring only 300 mm of precipitation per year, Opuntiacan produce a large amount of dry matter feedstock and still retain enough moisture to facilitate biogas production. It’s possible to get as much as 2.5 kWh of methane from 1 kg of dry Opuntia.

4. Phylogenetic Importance
Trained botanists and amateurs alike have held cacti in high regard for centuries. The copious production of spines, lack of leaves, bizarre architecture and impressive ability to persist in the harshest environments on Earth are all traits that have entitled this lineage to be named a true wonder of the plant world.

The cacti are one of the most celebrated radiations of succulent plants. There has been much speculation about their age, but progress in dating cactus origins has been hindered by the lack of fossil data for cacti or their close relatives. Through whole genome sequencing, we help will reveal the genomic evolution of Opuntia by comparing this genome with that of other sequenced plant species.

Cacti are typical CAM plants. We will analyse the evolution of CAM genes in the cactus to help reveal the secret of drought tolerance. Furthermore, plant architecture genes and MADS-box gene family members will be analysed to reveal the specific architecture and structure of cactus.

Crowdfunding the Cactus Genome Project
Cactus has several fascinating aspects that are worth exploring, not just for its biology, but also its relevance to humanity and the global environment. We plan to generate a draft genome for Opuntia, and have launched a crowdfunding campaign to help fund this project – we have already raised $2300 USD (46% of what we need), but we only have 15 days to raise the rest. If you would like to help fund this project, please visit our Experiment page at: https://experiment.com/projects/sequencing-the-cactus-genome-to-discover-the-secret-of-drought-resistance.

If we are successful in raising enough money to initiate the Cactus Genome Project, not only will this be the first plant genome to be sequenced in the Cactaceae family, we will be releasing the results to the plant science community through GeneGarden, an ornamental plant genome database. Our citizen science approach is also allowing us to reach out directly to members of the public, creating exciting opportunities for outreach and engagement with plant science.

If you have any further questions, please contact project leader Dr Peng Jiang at pjiang@uga.edu.

This blog post is slightly adapted from a post originally appearing on GigaScience Journal’s GigaBlog. Reproduced and adapted with permission, under a CC-BY license.

References

  1. Wolfram RM, Kritz H, Efthimiou Y, et al. Effect of prickly pear (Opuntia robusta) on glucose- and lipid-metabolism in non-diabetics with hyperlipidemia – a pilot study. Wien Klin Wochenscr. 2002;114(19–20):840–6.
  2. Trejo-Gonzalez A, Gabriel-Ortiz G, Puebla-Perez AM, et al. A purified extract from prickly pear cactus (Opuntia fulignosa) controls experimentally induced diabetes in rats. J Ethnopharmacol. 1996;55(1):27–33.
  3. Fernandez ML, Lin EC, Trejo A, et al. Prickly pear (Opuntia sp.) pectin alters hepatic cholesterol metabolism without affecting cholesterol absorption in guinea pigs fed a hypercholesterolemic diet. J Nutr. 1994;124(6):817–24.
  4. Frati-Munari AC, Gordillo BE, Altamirano P, et al. Hypoglycemic effect of Opuntia streptacantha Lemaire in NIDDM. Diabetes Care. 1988:11(1):63–66.

Access to crop seeds through an SMTA: what is that?

By | Blog, Future Directions

Carolina Roa, Independent Consultant at CropIP

“We need a material transfer agreement”

Copyright: CIAT, CC BY-SA.

© CIAT, licensed under Creative Commons CC BY-SA.

As a plant breeder in the area of food and agriculture you look for well-characterized ­– or at least well-referenced – plant materials suitable for making crosses and generating populations to be tested for agricultural traits. If you or your organization don’t already have such materials, you are likely to contact people at seed or germplasm banks, research or breeding programs to obtain sexual or vegetative seeds.

Have the entities come back to you saying that to get access to the plant material you and/or your organization need to agree to the terms of a material transfer agreement (MTA)? Have they perhaps used the expression “Standard Material Transfer Agreement (SMTA)”? Likewise, if you wanted to provide germplasm to a colleague or a breeder/researcher at another institute, has your own organization told you that an MTA or an SMTA is required? You may be asking yourself, “What is an MTA or SMTA, and why are they required?” This article aims to address these questions.

International and national contexts behind the agreements

Around 20 years ago, no written agreement was necessary to exchange plant materials used for research, breeding or training in the area of food and agriculture, particularly if one was working in the public sector. A verbal agreement was likely to suffice. The latter, however, meant that access to plant materials depended in great measure on personal or inter-organizational relationships, geographic proximity, reciprocity and mutual gain, and interactions between governments1.

Maize active collection. © Xochilquetzal-Fonseca, CIMMYT, licensed under Creative Commons CC BY-NC-SA.

Maize active collection. © Xochilquetzal Fonseca, CIMMYT, licensed under Creative Commons CC BY-NC-SA.

In the early 1990s the situation changed with the advent of two major international treaties. The Convention on Biological Diversity (CBD), in force since 1993, deals with access to all biological diversity, including all genetic resources, as well as the sharing of benefits arising from their use. The International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA; referred to as the Plant Treaty), in force since 2004, carved a niche for plant genetic resources for food and agriculture (PGRFA) and created a multilateral system to facilitate access and benefit sharing for PGRFA deemed important for food security2.

A large number of countries are members of one or both of these treaties, currently 194 countries in the case of the CBD and 134 in the case of the Plant Treaty. The country members (called Contracting Parties) have implemented national laws and regulatory measures to adopt and adapt these regimes at national levels. Implementation, however, is not uniform. Some countries have amended existing national laws to incorporate the main aspects of the international treaties. Others have issued specific national laws that reproduce the international instruments and have added aspects pertinent to their national contexts, and a number of countries have not yet implemented the treaties at a national level. Therefore, as a plant breeder/researcher you are likely to encounter different rules and conditions for accessing or providing PGRFA, depending on whether the country where the materials are located have implemented the CBD, the Plant Treaty, both or none, and depending on the rules and regulations applicable at the organizations hosting/administering the plant materials, including your own institute.

MTA or SMTA? What’s the difference?

ILRI Forage genebank_ILRI-Stevie Mann-CC BY-NC-SA

ILRI forage genebank © Stevie Mann, ILRI, licensed under Creative Commons CC BY-NC-SA.

Whether an MTA with specific conditions of access and use, or the SMTA with standardized conditions applies for the plant materials to be exchanged depends on whether CBD-derived regulations apply, or whether the Plant Treaty operates. In case CBD rules apply, you or your institute, as a prospective recipient, will receive an MTA with conditions defined by the germplasm provider. You/your institute will need to accept the terms as they are, or try negotiating and modifying them to suit your purposes. This process normally takes time and legal skills. Going through this bilateral negotiation process every time new plant material is requested from another entity could be a deterrent to the research, and breeding work might not progress at the pace and scale that is needed to address growing food security challenges.

The SMTA, as its name indicates, was designed by the negotiators of the Plant Treaty as a standard and multilateral MTA with fixed terms and conditions of access and use, applicable to plant materials from 64 food and feed crops listed in Annex 1 of the Plant Treaty, which are under public management and control and in the public domain. This system is referred to as the Multilateral System of access and benefit sharing (MLS for short). The SMTA also applies to PGRFA placed voluntarily into the MLS by its holders. Therefore, if particular PGRFA required for research, breeding and/or training purposes are under the MLS, the SMTA as it is applies without the need to negotiate terms, saving time and costs.

At this point it is worth clarifying that PGRFA, even belonging to the crops listed in Annex 1 of the Plant Treaty, owned or administered by private corporate entities are generally outside the MLS. Likewise, PGRFA of the listed crops growing in farmers’ fields, or PGRFA under development by breeders or farmers (that is, not ready for commercialization and commonly referred to as ‘breeding materials’) may not be under the MLS. Their inclusion in the MLS is at the discretion of the owner/holder, the grower or the developer of the breeding materials. If they place such materials under the MLS, they will need to use the SMTA as the instrument for access and benefit sharing for the purposes specified in the Plant Treaty. However, the developer is entitled to add terms and conditions to the SMTA.

How the SMTA works3

CIAT Genebank_Luigi Guarino_CC BY

CIAT genebank © Luigi Guarino, licensed under Creative Commons CC BY.

Scope of use – PGRFA under the SMTA can be used for research, breeding and/or training in the fields of food and agriculture. If the intended use is different, e.g., extraction of compounds to be used for chemical or pharmaceutical applications, the SMTA is not the instrument to use. Other conditions dictated by national legislation, the holder/owner of the resources, or both may apply for non-food/feed applications.

Facilitated access – access to PGRFA should be free of charge and expeditious, without the need to track individual accessions. If a fee is charged, it should reflect ‘minimal costs’ related to shipment and transport costs. For instance, costs of seed maintenance, seed production, and the like should not be included.

Provider’s obligations and rights – the main obligations of PGRFA providers include (1) granting ‘facilitated access’ to PGRFA and associated passport data and non-confidential descriptive information, and (2) reporting periodically to the Secretariat of the Plant Treaty about the SMTAs entered into. As a provider and developer of breeding materials, you will have discretion on granting access to such materials while they are under development. If you grant others access to such materials, you’d be entitled to add terms and conditions to the SMTA, including aspects such as payments, limitations on subsequent transfers, etc.

Recipient’s obligations and rights – the main obligations that come with materials received under the SMTA include: (1) to exclusively use them for research, breeding, and/or training related to food and agriculture; (2) to not claim intellectual property rights or any other rights that may limit facilitated access; (3) to use a new SMTA for subsequent transfers; and (4) to report such subsequent transfer to the Secretariat of the Plant Treaty.

If the recipient were to subsequently transfer PGRFA under development, the recipient will act as a provider and in this case, s/he should (1) use a new SMTA; (2) identify in Annex 1 of the new SMTA the material from which the breeding materials were derived; and (3) report this transaction to the Secretariat of the Plant Treaty.

If additional conditions are added to the SMTA for the transfer (or the subsequent transfer) of PGRFA under development, they should go as a separate agreement to the associated SMTA and there is no need to report such add-on conditions to the Secretariat of the Plant Treaty. A recipient of PGRFA, whether under development or not, has no further duties with respect to the actions of a subsequent recipient.

Benefit sharing commitments – As a recipient, you are expected to share the benefits obtained from MLS materials with the agricultural community in general. As an example, granting access for further research and breeding to products developed by incorporating MLS materials received, is one of such benefits. In this case, you may also voluntarily contribute funds to the Benefit Sharing Fund, administered by the Plant Treaty, which finances food and feed-related research projects, mostly in developing economies. Conversely, if you decided to restrict further access to your MLS-derived products, you would be required to pay to the Benefit Sharing Fund either 0.77% or 0.5% of the sales of your product, depending on whether you opted to pay per accession received (first amount) or per crop accessed (second figure). The payment requirement operates regardless of how much MLS-derived material has been incorporated into your product and it will last as long as access to the product is restricted.

Duration – the particular SMTA you entered into will be valid as long as the Plant Treaty remains in force.

Genebanks using the SMTA

Rice seed varieties. Copyright: IRRI CC BY-NC-SA 2.0

Rice seed varieties. © IRRI, licensed under Creative Commons CC BY-NC-SA 2.0.

Apart from national seed collections of member countries, there are international institutions that have placed their seed holdings under the purview of the Plant Treaty.  The International Agricultural Research Centers of the Consultative Group on International Agricultural Research (CGIAR) are among those institutions. Eleven of the CGIAR centers, holding approximately 700,000 accessions of crops listed in Annex 1, as well as non-Annex 1 crops and breeding materials, use the SMTA to transfer these materials for the purposes specified by the Plant Treaty.

The individual websites of the CGIAR centers publish lists of available accessions, and requests can be placed electronically. As a prospective recipient, you should receive confirmation of availability of sufficient seed for shipping together with an electronic copy of the SMTA. You have the options to accept the SMTA terms through a mouse click, by signature, or by ripping the package containing the seed and a printed copy of the SMTA. From this point onwards, the rights and obligations of the SMTA for both providers and recipients start operating.

Therefore, next time you receive an SMTA don’t despair; come back to these notes and seek guidance from the legal or other pertinent office at your organization on how to proceed with this or any other kind of agreement. ©

 

REFERENCES

  1. Halewood, M (2013). What kind of goods are plant genetic resources for food and agriculture? Towards the identification and development of a new kind of commons. International Journal of the Commons 7(2): 278–312.
  2. Moore, Gerard and Tymowski. 2005. Explanatory guide to the International Treaty on Plant Genetic Resources for Food and Agriculture. IUCN, Gland, Switzerland and Cambridge, UK. xii + 212 pp.
  3. Standard Material Transfer Agreement (accessed at http://www.planttreaty.org/content/what-smta).

 

About the author: Carolina Roa is plant biologist and a legal professional with around 25 years of experience. She has worked for the public and private sector in different parts of the world on a range of legal and intellectual-property aspects related to agriculture and biotechnology. Carolina is currently the Principal Consultant at CropIP. She can be reached at carolina@crop-ip.info.

Yes, Africa will feed itself within the next 15 years

By | Blog, Future Directions

Africa will be able to feed itself in the next 15 years. That’s one of the big “bets on the future” that Bill and Melinda Gates have made in their foundation’s latest annual letter. Helped by other breakthroughs in health, mobile banking and education, they argue that the lives of people in poor countries “will improve faster in the next 15 years than at any other time in history”.

Their “bet” is good news for African agriculture: agronomy and its natural twin, agricultural extension, are back on the agenda. If Africa is to feed itself, the women and men who grow its crops need access to technical expertise on how to manage their variable natural resources and limited inputs and market intelligence on what to grow, what to sell and what to keep.

New tools in the hands of farmers

The Gates foundation report outlines that African countries spend $50 billion a year importing food. Nigeria alone imports $500m of rice from Vietnam each year.

But there is no quick fix that will transform African agriculture without skillful agronomy and intelligent extension. Whatever the promises brought by new, drought-tolerant varieties of crops such as maize, they cannot achieve their potential without the wise management of fertilisers, timing of cultivations and appropriate crop rotations.

Bill & Melinda Gates Foundation 

As the graph above shows, sub-Saharan Africa’s crop yields remain very low compared to the rest of the world. Sadly, in our rush for only genetic solutions to increasing agricultural yields, we have ignored the fields and landscapes in which crops are grown. The consequence has been a missing generation of scientifically trained agronomists and agricultural extension workers – who help teach farmers about new farming practices – with the skill sets required to manage resources and apply principles.

Meanwhile, powerful tools such as geospatial mapping, predictive modelling, remote-sensing (using aerial imaging to assess the state of vegetation) and mobile technologies have advanced to a stage where they are of practical use to the scientific agronomist, educated extensionist and literate farmer. We now have a real opportunity to link genetic advances and improved management with the social and economic drivers for African agriculture. This “research value chain” between grower and consumer requires that each research discipline plays an interconnected role with the end-user always in sharp focus.

Soils and sustainability

So, what are the priorities for African agriculture in the next 15 years? First, we must rehabilitate its soils. Since 2015 has been declared as the UN International Year of Soil, we need to recognise that Africa has some of the world’s frailest soils, which have suffered most from “cereal abuse” through the almost continuous cultivation of cereal crops. These monocultures have left Africa’s soils tired and impoverished. Applications of fertilisers will not, by themselves, be enough to save them.

For sustainable agricultural systems, we need to reconsider our addiction to major cereals grown as monocultures and move from “calorie security” to “nutritional security”. For this, nitrogen-fixing leguminous crops have to be part of any solution. In his Noble Peace Prize address in 1970, Norman Borlaug, the father of what became known as the “green revolution” in South Asia, recognised the imbalance between research advances on the major cereals and those on all other crops:

The only crops which have been appreciably affected up to the present time are wheat, rice, and maize… nor has there been any appreciable increase in yield or production of the pulse or legume crops, which are essential in the diets of cereal-consuming populations.

Approaching 50 years later, the situation remains similar. Clearly, improvements in leguminous crops (such as beans and lentils), both in their own right as nutritious sources of food and as rehabilitators of soil, are long overdue. Since 2016 has been declared as the UN International Year of Pulses, there is no better opportunity to redress the historical imbalance noted by Borlaug.

Crops for the future

We also need to recognise that most African family farmers are women. Often the species they cultivate are not the major cash crops grown by men as mechanised monocultures. Rather, they are local “underutilised” species, often legumes and vegetables, which families cultivate in complex landscapes for their own sustenance.

These crops, and the multiple cropping systems which support them, have few influential champions and rarely feature in the research strategies of national and international agencies. But it is crops and agricultural systems such as these that will help Africa feed itself sustainably.

In a very real sense, these “crops for the future” will help diversify Africa’s agriculture to meet the volatile physical and economic climates that lie ahead. Unlike the major crops which have received billions of dollars of support over generations, underutilised crops deserve a “big bet” over the next 15 years if they are to help achieve major breakthroughs for most people in most poor countries.

The Conversation

This article was written by Sayed Azam-Ali, CEO of the Crops for the Future Research Centre and Professor of Global Food Security at University of Nottingham, and originally published at The Conversation.

Read the original article.

B.B. Singh’s quest to make cowpea the food legume of the 21st century

By | Blog, Future Directions

4fig3In 1944, the year Bir Bahadur (B.B.) Singh was born in the state of Uttar Pradesh in India, Indian agriculture was in shambles. During nearly 200 years of British rule, the country’s agricultural enterprise had been turned over to commodities such as cotton, indigo, and sugarcane for export; what little food was grown hinged on rainfall and the soil’s natural fertility—or lack of it. Crop yields were often abysmal as a result, and famine was common. So when India won independence from Britain in 1947, the Indian government enacted a sweeping program of nationwide, agricultural education.

That’s why when Singh graduated in 1956 from his village school with good grades and an interest in science, he found himself at one of India’s newly minted agricultural high schools. It was the only nearby school where he could study science, Singh says, as well as the closest high school to his home. Plus, his father wanted him to attend, saying, “Why don’t you study agriculture and see what help you can give to our people,” Singh recalls.

“So I was okay with going to an agricultural high school, and that later became my good luck,” he says. Turns out it also became the good luck of millions of the world’s smallholder farmers.

Today, Singh is among the most revered breeders of legume—or pulse—crops, credited with improving the diets, incomes, and lives of farming families across Africa, Asia, and South America. In the late 1960s and 1970s, for instance, the ASA and CSSA Fellow not only established the first systematic breeding program for soybean in India, but was also pivotal in bringing the novel food to millions of Indian people. Soybean production has since grown in India from just 5,000 tons in 1961 to about 12 million today. Yet this was only the start.

“Of course, B.B. is best known for his work with cowpea,” says Bill Payne, an ASA, CSSA, and SSSA Fellow who was at Texas A&M and CGIAR in Ethiopia before becoming dean of agriculture at the University of Nevada–Reno this winter. “Almost anywhere in the world, you cannot work on cowpea without running into him in some way, fashion, or form.”

imagesKnown also as black-eyed pea, cowpea is a staple crop in many tropical areas, and Singh’s signature achievement is a fast-maturing variety that fits into the rotational niches between wheat, maize, and rice. Due largely to this advance, worldwide cowpea production rose from 1.3 million to 7 million tons between 1981 and 2013—the only food legume to enjoy such an upswing. But the crop scientist, now in the 48th year of his career, isn’t content to stop there.

“I think there’s a very good possibility that we will have a surge in pulse production in the coming decades,” says Singh, who currently splits his time between Texas A&M University and India’s G.B. Pant University. The title of his new book, Cowpea—The Food Legume of the 21st Century asserts the same.

Those who know him don’t doubt it. “He’s just tenacious,” says CSSA President David Baltensperger, also an ASA and CSSA Fellow. He often compares Singh’s success with cowpea to Norman Borlaug’s accomplishments with wheat. “One of the secrets to B.B., like Dr. Borlaug, has been his ability to keep his eye on what he considers to be really powerful fundamentals. That leads to a lot of success over a long career.”

Good decisions… and a little luck

Focus is indeed crucial for a researcher, and other colleagues add that Singh is highly intelligent, full of energy, and a careful listener—as well as supremely dedicated to helping farmers.

“He is an excellent scientist—I mean, he publishes a lot,” says Ken Dashiell of the International Institute of Tropical Agriculture (IITA) in Ibadan, Nigeria, from which Singh retired in 2006. “But he probably spends 98% of his energy on getting the best cowpea varieties for the farmers, and 2% of his energy on publishing.”

What Singh himself says is that he’s been lucky. “At every stage of my life, some good people have come, given me direction, and good things have happened,” he says. The first stroke of luck came when his father pushed him toward an agricultural high school because it helped gain him admission in 1960 to India’s first agricultural university: Uttar Pradesh Agricultural University (now Pant University).

4fig3Singh then earned a scholarship in 1963 to do graduate studies in plant breeding at the University of Illinois, where again he made a fateful choice. After learning how much research was already under way to improve cereals, Singh resolved to study legumes to help India’s vegetarian multitudes meet their need for protein. And at the University of Illinois, that meant one option: soybean.

“So, that’s how I decided to work on soybean,” he says, “and it was one of the best decisions that I took in my life.”

Soybean contains roughly twice the protein of other pulses, he explains, and by the time he earned his Ph.D., USAID and the University of Illinois were already trying to bring soybean to countries beset by malnutrition, including India. Meanwhile, the dean of agriculture at Pant University was monitoring Singh’s progress, and in 1968 sent him a “very personal and emotional letter,” Singh says. It offered him—now a postdoc at Cornell—an assistant professorship at Pant that included 50% more salary than what a new assistant professor in India typically earned.

Singh had two competing offers from U.S. universities for substantially higher pay, but he never gave the decision a second thought. Later that year, he returned to India to begin the work that would transform soybean from an agricultural novelty into one of the nation’s principal foods.

He might have stayed at Pant for the rest of his career. But in 1977, a change in university administration led to major campus unrest, including the shooting of several staff. Hoping to get away for a “breathing spell,” Singh began looking for other opportunities and was immediately offered soybean breeding positions by the United Nation’s Food and Agriculture Organization (FAO) in Zambia and by IITA in Nigeria. Opting for IITA because of his interest in research, he intended to stay abroad for just two years, but “then based on my work, they kept me there forever, and I spent my life there,” he says.

They asked something else of him, as well: to work not on soybean, but cowpea.

Continue reading this story in the Oct. 2014 issue of CSA News magazine…

This blog was first published by the American Society of Agronomy

https://www.agronomy.org/science-news/bb-singhs-quest-make-cowpea-food-legume-21st-century

Cellulosic Ethanol from Sugarcane in Brazil

By | Blog, Future Directions, Global Change

sugarcane fieldBrazil is a major producer of ethanol from sugarcane, and this leading global position is the fruit of scientific and technological advances resulting from a development program that was initiated in the 1970s. Driven by the oil crises of 1972-1973, Brazil transformed several sugar mills into ethanol producing units that became capable of co-production of ethanol and raw sugar (5). This was technically possible due to the high levels of sucrose in sugarcane and to the development of yeast strains capable of fermenting this sugar efficiently. At the same time, the first automobiles running exclusively on ethanol were introduced, which on the one hand helped Brazil face major world energy crises, and on the other implanted the basis for development of future technologies. Over the following 40 years, Brazilian sugar mills undertook a technological transformation that significantly increased the efficiency of sucrose and alcohol production. This method, now called first generation (1G), has reached a level of 90% conversion of sucrose into ethanol (5). At the same time, advances in sugarcane agricultural technology improved the sugarcane crop to a high level of productivity (averaging 80 tones per hectare). Using intensive breeding programs, a number of sugarcane varieties have been developed that are increasingly better adapted to the diverse climate and soils encountered in Brazil. The result is that Brazil is now the second largest producer of ethanol and the first placed producer of sugarcane in the world.

The necessity to produce second-generation ethanol

Until 2006, Brazil was the only country to produce and use ethanol on a large scale as a fuel alternative for cars. Since then, increased public awareness and governmental focus around the world on issues related to climate change and the excessive use of fossil fuels has led to increased interest in the use of renewable energy. It was at this moment that Brazil, with its highly efficient sugarcane bioethanol sector, became a leader worldwide in the production and use of renewable energy. Nevertheless, production of 1G bioethanol was already at the limit of efficiency both from industrial and agronomical viewpoints.

It was in this context that the Brazilian scientific community and the Federal and State of São Paulo governments took the initiative in the search for ways to increase production of sugarcane ethanol beyond current limits. An idea that was already being revived in several places in the world was the possibility to produce ethanol from sugar polymers, including cellulose, present in cell walls of plants. This search for ‘cellulosic ethanol’ is generally referred to as second-generation (2G) ethanol. Although establishment of 1G technology was highly successful, the potential for ethanol production from 2G is much higher because energy accumulated in sugarcane in the form of sucrose represents only 1/3 of the total. The other two-thirds are distributed equally between the bagasse (stems) and the leaves.

Cell wall recalcitrance

At first sight, the idea of producing ethanol from biomass seems straightforward: it would be enough to convert cellulose to free sugars that could be fermented by yeast. Although many advances have been made in this area, this problem is far from being solved, and developing 2G processes that are economically viable has proven to be a major challenge. The plant cell wall is composed mainly of carbohydrates in the form of polysaccharides that associate to form a supramolecular structure where polymers aggregate through non-covalent linkages. Some polysaccharides are branched with phenolic compounds (ferulic an p-coumaric acids). Ferulic acid can dimerize interlocking polysaccharide chains or these can still undergo polymerization with other phenylpropanoids, including p-hydrocinammic, sinapyl and coniferyl alcohols, forming lignin. Together, the supramolecular structure of cell-wall polymers constitute the main obstacle to enzymatic hydrolysis. Furthermore, known hydrolytic enzymes have molecular sizes that prevent their penetration into the polymer matrix. Therefore, when a mixture of enzymes is added to the surface of the cell wall, the catalytic attack is mainly on the surface of the composite. To perform more complete hydrolysis, enzymatic complexes would have to act in a synergetic fashion on the entire cell wall composite. At present this is not feasible as researchers cannot adequately control the process because very little is known about the synergism between the enzymes involved. One of the principal limitations to understand such mechanisms is that until recently our knowledge of the structure and architecture of the sugarcane cell wall was very limited.

Sugarcane buckAt the biological level, cell wall recalcitrance in plants is thought to be due to the wall’ ability to protect against herbivores and the penetration of pathogens. At the molecular level, the cell wall of sugarcane presents three domains of polysaccharides that interact through non-covalent linkages: the pectic domain, the hemicellulosic domain and the cellulosic domain. The cellulosic domain is embedded within the hemicellulosic domain and both are embedded in the pectin domain. Thus, the basic unit of the cell wall of sugarcane consists of a core with macrofibrils (agglomerated of microfibrils) of cellulose strongly linked to structurally complex hemicelluloses that display a glycomic code, the complex branching pattern of these compounds (2). In addition, this core of polysaccharides is surrounded by an agglomerate of polymers that interact with themselves. Phenolic compounds are also thought to interlock the three polysaccharide domains so that the covalent linkages are protected, effectively sealing the whole unit and creating a structure that is extremely resistant to mechanical, chemical and biochemical degradation.

Several publications produced by the research labs of the National Institute of Science and Technology of Bioethanol (INCT-Bioetanol – www.inctdobioetanol.com.br) have demonstrated that it is possible to disassemble the cell wall using chemical reagents (4). The procedure consists of initially attacking the phenolic compounds and eliminating them from the wall. This makes subsequent separation of the wall polysaccharides possible via treatment with a series of alkali solutions of increasing concentration (6).

A procedure called pretreatment (chemical and physical treatments with hot water, ammonia, acids and/alkali), eliminates the porosity barrier so that all polymers become accessible to attack by hydrolases. However, the branching nature of hemicelluloses still acts as a barrier and prevents further enzyme attack of the polymer chains. This highlights the necessity of using specific enzymatic complexes in order to produce free sugars that can be utilized for fermentation (1-7). As branched hemicelluloses alter the way polysaccharides are recognized by enzymes, their branching pattern (glycomic code) can alter the interaction between enzyme and substrate, affecting enzyme kinetics and cell wall degradation efficiency. The available data shows that the cell wall of sugarcane displays at least 18 glycosidic linkages, and suggests that approximately the same number of enzymes will be necessary to degrade the cell wall completely (5,6). Nevertheless, this chemical process is extremely complicated, laborious and expensive, and this is therefore not a viable strategy for industry.

The collection of enzymes characterized during the first phase of the INCT-Bioetanol contains practically all the catalytic capabilities needed for complete sugarcane cell wall hydrolysis. For this reason, the Institute has reached a point of prioritizing experiments focused on combining enzymes, forming consortia capable of dealing with each of the limiting factors related to recalcitrance. The possible combinations of enzymes have been proposed (1,6) and during the next phase of the project, these strategies will be put into practice by an integrated group of researchers in a series of experiments that will test this hypothesis.

At the same time, it will be necessary to understand the variability in the structure of the sugarcane cell wall in order to find Brazilian sugarcane varieties possessing structures and architectures that are more amenable to hydrolysis. Although the variation in cell wall composition is relatively limited among sugarcane tissues, one may expect to find considerable variation among the great number of extant varieties. This has been recently observed for Miscanthus and maize, two grass species that are genetically related to sugarcane and with very similar cell walls. Several research groups have concentrated efforts on understanding the role of lignin in recalcitrance and have concluded that this interference is somewhat limited. The reduction in lignin content leads in general to an increase in saccharification in a non-linear fashion depending on the pre-treatment, morphological distribution and the level of lignin aggregation (9), suggesting that other cell wall domains make equally important contributions to the recalcitrance of biomass. Research groups of the INCT-Bioetanol have already obtained transformed sugarcane in which the gene encoding one of the enzymes of lignin biosynthesis (COMT) has been silenced. These transgenic plants have cell walls that are modified, and saccharification tests are currently in progress. During the second phase of the INCT we intend to verify whether such genetic variability also exists in sugarcane and to use this information to obtain varieties in which differences among cell wall composition lead to lower recalcitrance to hydrolysis.

 

Marcos S. Buckeridge

msbuck@usp.br

Laboratory of Plant Physiological Ecology, Depatment of Botany, Institute of Biosciences, University of São Paulo (www.lafieco.com.br)

Director of the National Institute of Science and Technology of Bioethanol (www.inctdobioetanol.com.br)

 

REFEFENCES

  1. Buckeridge, M.S., Dos Santos,W.D., Tiné, M.A.S., De Souza, A.P. (2015) Compendium of Bioenergy Crops: Sugarcane edited by Eric Lam. CRC Press, Taylor and Francis (in press)
  2. Buckeridge, M.S. & De Souza, A.P. (2014) Breaking the “glycomic code” of cell wall polysaccharides may improve second generation bioenergy production from biomass. Bioenergy Research DOI 10.1007/s12155-014-9460-6
  3. Buckeridge, M.S.; Souza, A.P.; Arundale, R.A.; Anderson-Teixeira, K.J.; DeLucia, E. (2012) Ethanol from sugarcane in Brazil: a “midway” strategy for increasing ethanol production while maximizing environmental benefits. GCB Bioenergy, 4:119-126.
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  5. De Souza, A. P. ; Grandis, A. ; Leite, D. C. C. ; Buckeridge, M.S. (2014) Sugarcane as a Bioenergy Source: History, Performance, and Perspectives for Second-Generation Bioethanol. Bioenerg Res, 7:24-35.
  6. De Souza, A. P., Leite, D. C. C., Pattathil, S. ; Hahn, M. G. ; Buckeridge, M. S. (2013) Composition and Structure of Sugarcane Cell Wall Polysaccharides: Implications for Second-Generation Bioethanol Production. Bioenergy Research, 6: 564-579.
  7. Mccann, M. ; Buckeridge, M. S. ; Carpita, N.C. . Plants and Bioenergy. 1. ed. New York: Springer, 2013. v. 1. 300p.
  8. Magrin, G.O., J.A. Marengo, J.-P. Boulanger, M.S. Buckeridge, E. Castellanos, G. Poveda, F.R. Scarano, and S. Vicuña, 2014: Central and South America. In: Climate Change 2014: Impacts, Adaptation, and Vulnerability. Part B: Regional Aspects. Contribution of Working Group II to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change [Barros, V.R., C.B. Field, D.J. Dokken, M.D. Mastrandrea, K.J. Mach, T.E. Bilir, M. Chatterjee,K.L. Ebi, Y.O. Estrada, R.C. Genova, B. Girma, E.S. Kissel, A.N. Levy, S. MacCracken, P.R. Mastrandrea, and L.L. White (eds.)]. Cambridge University Press, Cambridge, United Kingdom and New York, NY, USA, pp. XXX-YYY
  9. Rezende, C.A.; Lima, M.; Maziero, P.; Azevedo, E.; Garcia, W.; Polikarpov, I. (2011) Chemical and morphological characterization of sugarcane bagasse submitted to a delignification process for enhanced enzymatic digestibility. Biotechnology for Biofuels. 4: 54