Login

GPC Members Login
If you have any problems or have forgotten your login please contact [email protected]


Plant peptide helps roots to branch out in the right places

How do plants space out their roots? A Japanese research team has identified a peptide and its receptor that help lateral roots to grow with the right spacing. The findings were published in the online edition of Developmental Cell.

The team was led by Professor Hidehiro Fukaki (Graduate School of Science, Kobe University), Researcher Koichi Toyokura (currently JSPS Research Fellow at Osaka University) and Project Assistant Professor Tatsuaki Goh (currently Assistant Professor at the Nara Institute of Science and Technology) in collaboration with Professor Yoshikatsu Matsubayashi and Assistant Professor Hidefumi Shinohara (both from Nagoya University) and other researchers from the Nara Institute of Science and Technology, Associated Professor Koichi Fujimoto (Osaka University) and Assistant Professor Yuki Kondo (the University of Tokyo).

Plant root systems are mainly shaped by the lateral roots that grow from tissue inside the existing roots. These roots form from “lateral root founder cells” that are positioned at regularly-spaced intervals at a distance from the meristem tissue (tissue responsible for growth). Previous studies using Arabidopsis plants showed that lateral root founder cells are made from sites where there is high response to the chemical auxin, and indicated that transcription factor LBD16 induced by auxin may inhibit the cells near lateral root founder cells from forming roots.

This time a joint research team, using plant model Arabidopsis, searched for the gene that is activated by transcription factor LBD16 and successfully identified the TOLS2 gene. The TOLS2 gene is mainly expressed in lateral root founder cells and root germs. In Arabidopsis plants that overexpress TOLS2, the number of lateral roots decreases, indicating that the TOLS2 gene can inhibit the formation of lateral root founder cells. The team analyzed secretions from plants with overexpression of TOLS2 and revealed that the mature TOLS2 peptide is formed from 11 amino acids. When they artificially created mature TOLS2 peptide and added it to a wild-type Arabidopsis, the number of lateral root founder cells and lateral roots decreased.

Based on further investigation, the research team identified the receptor for TOLS2 as RLK7. RLK7 proteins express in the inner sheath of the roots (where the lateral root founder cells are located), the endodermis and the dermal layer, but RLK7 expression could not be found in the lateral root founder cells. It is likely that these proteins suppress the formation of lateral roots in cells adjacent to lateral root founder cells.

Next, using CRISPR/Cas9 genome editing technology, the team investigated how lateral roots form in other genetically-altered plant samples. Their results confirmed that the TOLS2 peptide and the RLK7 receptor are necessary to preserve the correct spacing between lateral root founder cells. From this analysis the research team proposed that Arabidopsis, by responding to auxin and inducing TOLS2 peptide in lateral root founder cells, through RLK7 receptors inhibits nearby lateral root founder cells in a non-cell-autonomous manner.

Professor Fukaki comments: “If the mechanism for TOLS2 peptide-based inhibition of nearby lateral root founder cells is clarified in Arabidopsis, this will help us to understand root formation mechanisms in other plants such as crops and trees. And if other plants contain peptides that fulfil the same function as the TOLS2 peptide, we could potentially use this mechanism to artificially control root formation patterns for crops and trees.”

Read the paper: Developmental Cell

Article source: KOBE University

Image credit: Developmental Cell

News

A small number of crops are dominating globally. And that’s bad news for sustainable agriculture

A new University of Toronto study suggests that globally we're growing more of the same kinds of crops, and this presents major challenges for agricultural sustainability on a global scale.


How plants cope with iron deficiency

Iron is an essential nutrient for plants, animals and also for humans. It is needed for a diverse range of metabolic processes, for example for photosynthesis and for respiration. If a person is lacking iron, this leads to a major negative impact on health. Millions of people around the globe suffer from iron deficiency each year. Iron enters the human food chain through plants, either directly or indirectly. Although there are large quantities of iron in the soil in principle, plants may become iron-deficient because of the specific composition of the soil. Additionally, a plant's iron requirements vary throughout its development depending on external circumstances.


Biotechnology to the rescue of Brussels sprouts

An international team has identified the genes that make these plants resistant to the pathogen that attacks crops belonging to the cabbage family all over the world.